It should be borne in mind that the preparation, depending on the supplementation time, may bring various effects. Mainly after 2-4 hours from application the feeling of sleepiness may appear and the increase of the tendency to fall asleep. Taking melatonin during the day may cause the feeling of sleepiness and body temperature reduction, while taking it in early evening contributes to accelerating the time needed to fall asleep, sleep latency reduction (the estimated time of waiting for sleep) and REM phase.

In case of the lack of problems with falling asleep, but when there are problems with waking up during the night, is it claimed that melatonin should be taken immediately before going to sleep or immediately after waking up at night, while in the situation of problems with falling asleep, the capsule should be taken an hour or even 2 hours before bedtime.

It has been observed that melatonin supplementation around 8-13 hours before the appearance of the lowest body temperature has a stimulating effect on the pace of biological rhythms, i.a. of sleep-wakefulness, prolactin, body temperature and melatonin. Taking the hormone around 1-4 hours after the lowest body temperature, on the other hand, inhibits the course of the above mentioned phases.